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| Registros recuperados: 42 | |
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| Boulais, Myrina; Soudant, Philippe; Le Goic, Nelly; Quere, Claudie; Boudry, Pierre; Suquet, Marc. |
| In the Pacific oyster, spermatozoa are characterized by a remarkably long movement phase (i.e., over 24 h) sustained by a capacity to maintain intracellular ATP level. To gain information on oxidative phosphorylation (OXPHOS) functionality during the motility phase of Pacific oyster spermatozoa, we studied 1) changes in spermatozoal mitochondrial activity, that is, mitochondrial membrane potential (MMP), and intracellular ATP content in relation to motion parameters and 2) the involvement of OXPHOS for spermatozoal movement using carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The percentage of motile spermatozoa decreased over a 24 h movement period. MMP increased steadily during the first 9 h of the movement phase and was subsequently maintained at a... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Intracellular ATP content; Mitochondrial membrane potential; Oxidative phosphorylation; Sperm motility. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00303/41376/40666.pdf |
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| Dudognon, Tony; Guderley, Helga; Quere, Claudie; Soudant, Philippe; Racotta, Ilie; Kraffe, Edouard. |
| Although laboratory experiments allow greater control of environmental conditions than field studies, they have several drawbacks. To analyze physiological responses to forcing environmental variables, experimental conditions should mimic natural conditions as closely as possible. For filter-feeding organisms in particular, diet quality and quantity is one of the environmental parameters that can differ markedly between experimental and field conditions. In the hatchery, Pacific oysters, Crassostrea gigas, commonly show good physiological performance and growth on a mixed algal diet of Tisochrysis lutea, formerly Isochrysis aff. galbana clone Tahiti (T-Iso), and Chaetoceros calcitrans, presumably as it provides a good supply of essential polyunsaturated... |
| Tipo: Text |
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| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00266/37756/36065.pdf |
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| Fuhrmann, Marine; Richard, Gaëlle; Quere, Claudie; Petton, Bruno; Pernet, Fabrice. |
| Environmental change in the marine realm has been accompanied by emerging diseases as new pathogens evolve to take advantage of hosts weakened by environmental stress. Here we investigated how an exposure to reduced seawater pH influenced the response of the oyster Crassostrea gigas to an infection by the Ostreid herpesvirus type I (OsHV-1). Oysters were acclimated at pH 8.1 or pH 7.8 and then exposed to OsHV-1. Their survival was monitored and oyster tissues were sampled for biochemical analyses. The survival of oysters exposed to OsHV-1 at pH 7.8 was lower (33.5%) than that of their counterparts at pH 8.1 (44.8%) whereas levels of OsHV-1 DNA were similar. Energetic reserves, fatty acid composition and prostaglandin levels in oyster did not vary... |
| Tipo: Text |
Palavras-chave: Acidification; Bivalve; Disease; Environment; Metabolism; Mortality risk. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00472/58334/60887.pdf |
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| Pernet, Fabrice; Malet, Nathalie; Pastoureaud, Annie; Vaquer, Andre; Quere, Claudie; Dubroca, Laurent. |
| Carbon stable isotopes and fatty acids were measured in the suspended particulate organic matter (POM) of the Thau lagoon to study its qualitative temporal changes in relation to environmental factors and to identify the food sources of bivalves over a one-yr-cycle in relation to their growth. Reciprocally, the impact of shellfish farming on POM was also studied. Oysters and mussels were sampled and measured for biometry, stable isotopes and fatty acid composition. Water samples were collected at two sites, both inside and outside of the shellfish farming area, to determine concentrations in POM, chlorophyll a (Chl a) and stable isotopes. Carbon isotopes and fatty acids in bivalves reflected seasonal changes in food sources, which varied consistently with... |
| Tipo: Text |
Palavras-chave: Aquaculture; Bivalve; Competition; Feeding ecology; Growth; Trophic markers. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00075/18575/16177.pdf |
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| Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Malet, Nathalie; Pastoureaud, Annie; Munaron, Dominique; De Lorgeril, Julien; Bachere, Evelyne; Vaquer, Andre; Huvet, Arnaud; Corporeau, Charlotte; Normand, Julien; Boudry, Pierre; Moal, Jeanne; Quere, Claudie; Quilien, Virgile; Daniel, Jean-yves; Pepin, Jean-francois; Saulnier, Denis; Gonzalez, Jean-louis. |
| This study was conducted in the Mediterranean area during a mass mortality event of Pacific oyster Crassostrea gigas. The objectives of this work were to increase our understanding of the mass mortality phenomena in Pacific oysters, to identify risky cultivation practises and to propose mitigation strategies. In the first part of this work, we followed environmental parameters of the Thau lagoon in relation with mortality events. Then, we compared mortality and growth parameters of oysters as a function of their origins, their development stages and cultivation sites in relation with energetic, reproduction and pathogens. Finally, we conducted transfer experiments where oysters maintained free of mortality in open sea were transferred in the Thau lagoon... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11354/7927.pdf |
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| Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Lagarde, Franck; Fiandrino, Annie; Huvet, Arnaud; Corporeau, Charlotte; Boudry, Pierre; Quere, Claudie; Degremont, Lionel; Pepin, Jean-francois; Saulnier, Denis; Boulet, Hélène; Keck, Nicolas. |
| The objectives of this study are to increase our understanding of the mass mortality phenomena in Pacific oysters Crassostrea gigas in the Thau lagoon and to examine the effect of cultivation practises. Mortality events in 2009 and 2010 always coincide with simple or dual infections involving primarily the Herpes virus OsHV-I and secondarily the bacteria Vibrio splendidus, when seawater temperature is between 17°C and 24°C. In the Thau lagoon, mortality starts early May and last until fall with an interruption period when seawater temperature is higher than 24°C in July and August. Mortality of oysters varies as a function of zootechnical life-history, hydrodynamics and cultivation practises. First, mortality depends on whether or not oysters have been... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15404/12764.pdf |
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| Corporeau, Charlotte; Vanderplancke, Gwenaelle; Boulais, Myrina; Suquet, Marc; Quere, Claudie; Boudry, Pierre; Huvet, Arnaud; Madec, Stephanie. |
| We used a 2-DE proteomic approach to identify abundant proteins linked to oocyte quality in the Pacific oyster Crassostrea gigas, an economically important bivalve. Oocyte quality of 14 females was estimated by recording fertilisation and early developmental success until D-larval stage under controlled conditions. Proteins that were differentially expressed between females showing high or low oocyte quality were identified by nano-liquid chromatography tandem mass spectrometry. Twelve up-accumulated spots associated with low quality oocytes revealed 10 distinct proteins, including vitellogenin — breakdown products and metabolic enzymes. Eight up-accumulated spots from high quality oocytes revealed 6 distinct proteins, including chaperone molecules and... |
| Tipo: Text |
Palavras-chave: Marine bivalves; Crassostrea gigas; Oocyte quality; Proteomics. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00098/20925/18534.pdf |
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| Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie. |
| Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters... |
| Tipo: Text |
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf |
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| Guevelou, Eric; Huvet, Arnaud; Sussarellu, Rossana; Milan, Massimo; Guo, Xinmei; Li, Li; Zhang, Guofan; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte. |
| AMP-activated protein kinase α (AMPKα) is a key regulator of energy balance in many model species during hypoxia. In a marine bivalve, the Pacific oyster Crassostrea gigas, we analyzed the protein content of adductor muscle in response to hypoxia during 6 h. In both smooth and striated muscles, the amount of full-length AMP-activated protein kinase α (AMPKα) remained unchanged during hypoxia. However, hypoxia induced a rapid and muscle-specific response concerning truncated isoforms of AMPKα. In the smooth muscle, a truncated isoform of AMPKα was increased from 1 to 6 h of hypoxia, and was linked with accumulation of AKT kinase, a key enzyme of the insulin signaling pathway which controls intracellular glucose metabolism. In this muscle, aerobic metabolism... |
| Tipo: Text |
Palavras-chave: Marine bivalve; Crassostrea gigas; Hypoxia; AMP-activated protein kinase; Alternative splicing; Adductor muscle. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00119/22998/21397.pdf |
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| Guevelou, Eric; Huvet, Arnaud; Galindo-sanchez, Clara E.; Milan, Massimo; Quillien, Virgile; Daniel, Jean-yves; Quere, Claudie; Boudry, Pierre; Corporeau, Charlotte. |
| The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the... |
| Tipo: Text |
Palavras-chave: AMP-activated protein kinase; Crassostrea gigas; Gametogenesis; Marine bivalve; Reproduction. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00168/27911/26470.pdf |
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| Pernet, Fabrice; Lagarde, Franck; Jeannee, Nicolas; Daigle, Gaetan; Barret, Jean; Le Gall, Patrik; Quere, Claudie; Roque D'Orbcastel, Emmanuelle. |
| Although spatial studies of diseases on land have a long history, far fewer have been made on aquatic diseases. Here, we present the first large-scale, high-resolution spatial and temporal representation of a mass mortality phenomenon cause by the Ostreid herpesvirus (OsHV-1) that has affected oysters (Crassostrea gigas) every year since 2008, in relation to their energetic reserves and the quality of their food. Disease mortality was investigated in healthy oysters deployed at 106 locations in the Thau Mediterranean lagoon before the start of the epizootic in spring 2011. We found that disease mortality of oysters showed strong spatial dependence clearly reflecting the epizootic process of local transmission. Disease initiated inside oyster farms spread... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00181/29249/27663.pdf |
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| Huvet, Arnaud; Jeffroy, Fanny; Daniel, Jean-yves; Quere, Claudie; Le Souchu, Pierrick; Van Wormhoudt, A.; Boudry, Pierre; Moal, Jeanne; Samain, Jean-francois. |
| In the oyster Crassostrea gigas consumption-related traits, amylase properties and growth were found to be linked through genotypes that differed for polymorphism in the two amylase genes AMYA and AMYB. Modulation of AMYA mRNA level had already been observed in response to food availability, whereas the functional role of AMYB was still unknown. To improve knowledge about the regulation of amylase expression in C. gigas and the respective roles of the two genes, we made an assay of amylase expression at mRNA and enzymatic levels in the digestive gland of oysters that had received dietary supplements of starch. After 18 days, a significant increase of translatable mRNA for AMYB was observed, with a correlated increase in Michaelis-Menten constant Km values... |
| Tipo: Text |
Palavras-chave: Alpha-amylase; Crassostrea gigas; Digestive enzyme; Michaelis-Menten constant; Regulation. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00087/19869/17521.pdf |
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| Bechemin, Christian; Soletchnik, Patrick; Polsenaere, Pierre; Le Moine, Olivier; Pernet, Fabrice; Protat, Martin; Fuhrmann, Marine; Quere, Claudie; Goulitquer, Sophie; Corporeau, Charlotte; Renault, Tristan; Lapegue, Sylvie; Travers, Marie-agnes; Morga, Benjamin; Garriques, Manon; Garcia, Celine; Haffner, Philippe; Dubreuil, Christine; Faury, Nicole; Baillon, Laury; Baud, Jean-pierre. |
| Suite aux surmortalités observées au sein des élevages de moules touchant à la fois les animaux adultes et les juvéniles au mois de mars, dans les Pertuis Charentais, la Direction Générale de l’Alimentation (DGAl) a adressé une saisine à Ifremer le 04 avril 2014. Celle-ci fait état d’une demande d’appui scientifique et technique d’Ifremer pour tenter d’apporter des éléments de réponse par rapport à cette mortalité subite et importante. L’Ifremer a proposé un projet d’action permettant l’expertise du phénomène de surmortalité de la moule bleue, selon 3 axes : l’impact environnemental, la pathologie et les caractéristiques physiologiques des moules. Il s’agit de mettre en évidence la présence ou non d’agents infectieux connus ou émergents, d’apporter des... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00229/34022/32387.pdf |
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| Seguineau, Catherine; Soudant, P; Moal, Jeanne; Delaporte, Maryse; Miner, Philippe; Quere, Claudie; Samain, Jean-francois. |
| The present study tested two techniques for dietary supplementation of Crassostrea gigas spat with PUFA, such as arachidonic acid (AA). The first technique consisted of a preliminary enrichment and growth of an algal concentrate (T-ISO, Isochrysis sp.) with AA dissolved in an ethanol solution, the whole culture then being fed to the spat. This enrichment increased the AA weight percentage in T-ISO neutral and polar lipids from 0.6 to 22.4% and from 0.4 to 6.8%, respectively. The second delivery technique was direct addition separately of free AA dissolved in ethanol solution and algal concentrate (T-ISO + AA) to the spat-rearing tank. To test the efficiency of these delivery techniques, oyster spat were supplemented with AA-enriched T-ISO, T-ISO + AA, and... |
| Tipo: Text |
Palavras-chave: Food supply; Arachidonic acid; Spat; Crassostrea gigas; Dietary. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-1094.pdf |
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| Marchetti, Julie; Da Costa, Fiz; Bougaran, Gael; Quere, Claudie; Soudant, Philippe; Robert, Rene. |
| The lipid class and the fatty acid compositions of microalgae highly influence bivalve larval and post-larval development. Light is an essential environmental factor for microalgal culture, and quantity and quality of light may induce changes in the biochemical composition of the algae. The objective of this study was to investigate the effect of light spectrum (blue vs. white light) on lipid class and fatty acid compositions of Tisochrysis lutea cultured in a chemostat. Two different dilution rates (D) were assayed for each light spectrum: 0.2 and 0.7 day−1. Triacylglycerol (TAG), sterol, and hydrocarbon (HC) content increased sharply at low D. The proportion of alkenones was significantly reduced under blue light. Polyunsaturated fatty acids (PUFA), and... |
| Tipo: Text |
Palavras-chave: Tisochrysis lutea; Blue light; Dilution rate; Lipid classes; Fatty acids; Chemostat. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00412/52299/53355.pdf |
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| Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian. |
| Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol. Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf |
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| Gonzalez-araya, Ricardo; Lebrun, Luc; Quere, Claudie; Robert, Rene. |
| Four microalgae species (Rhodomonas salina, Thalassiosira weissflogii, Thalassiosira pseudonana and Pavlova lutheri) were evaluated to estimate their potential as food for Ostrea edulis (L.) reproductive conditioning. Best ingestion and absorption were observed with R. salina (3.44 and 1.59 mg g− 1 h− 1, respectively), followed by T. pseudonana (2.75 and 0.98 mg g− 1 h− 1) and P. lutheri (2.40 and 0.91 mg g− 1 h− 1). Oysters fed T. weissflogii exhibited the lowest ingestion and absorption values (1.40 and 0.68 mg g− 1 h− 1). Proximate composition (proteins and carbohydrates) and lipid content (fatty acids and sterols) analysed in four main tissues (gonad, digestive gland, muscle and gills) also differed significantly with diet. Protein ranged from 355 mg... |
| Tipo: Text |
Palavras-chave: Ostrea edulis; Conditioning; Algal diets; Ingestion; Absorption; Biochemical composition. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00090/20092/17731.pdf |
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| Delisle, Lizenn; Fuhrmann, Marine; Quere, Claudie; Pauletto, Marianna; Pichereau, Vianney; Pernet, Fabrice; Corporeau, Charlotte. |
| Voltage-dependent anion channel (VDAC) is a key mitochondrial protein. VDAC drives cellular energy metabolism by controlling the influx and efflux of metabolites and ions through the mitochondrial membrane, playing a role in its permeabilization. This protein exerts a pivotal role during the white spot syndrome virus (WSSV) infection in shrimp, through its involvement in a particular metabolism that plays in favor of the virus, the Warburg effect. The Warburg effect corresponds to an atypical metabolic shift toward an aerobic glycolysis that provides energy for rapid cell division and resistance to apoptosis. In the Pacific oyster Crassostrea gigas, the Warburg effect occurs during infection by Ostreid herpesvirus (OsHV-1). At present, the role of VDAC in... |
| Tipo: Text |
Palavras-chave: Voltage-dependent anion channel; Warburg effect; Crassostrea gigas; Ostreid herpes virus. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00420/53129/54142.pdf |
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| Registros recuperados: 42 | |
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